RESUMEN
Messenger RNAmRNAmedicine was urgently approved in 2020 as a vaccine for COVID-19 . However, current mRNA therapeutics are not fully established, with challenges remaining in translation efficiency and drug delivery system. Therefore, further research is needed to adapt mRNA therapeutics to other diseases. Furthermore, the preparation of mRNA drugs is time-consuming and costly because of the biological methods used. Our laboratory has been working on chemical methods to solve these issues. In this paper, we introduce chemical modifications and novel capping reactions as a method to improve the translation efficiency of mRNA and the introduction of disulfide modification to oligonucleotide therapeutics as an effort on the drug delivery system.Copyright © 2023, Japan Society of Drug Delivery System. All rights reserved.
RESUMEN
Messenger RNA(mRNA)medicine was urgently approved in 2020 as a vaccine for COVID-19 . However, current mRNA therapeutics are not fully established, with challenges remaining in translation efficiency and drug delivery system. Therefore, further research is needed to adapt mRNA therapeutics to other diseases. Furthermore, the preparation of mRNA drugs is time-consuming and costly because of the biological methods used. Our laboratory has been working on chemical methods to solve these issues. In this paper, we introduce chemical modifications and novel capping reactions as a method to improve the translation efficiency of mRNA and the introduction of disulfide modification to oligonucleotide therapeutics as an effort on the drug delivery system.Copyright © 2023, Japan Society of Drug Delivery System. All rights reserved.
RESUMEN
Recently messenger RNA （mRNA）therapeutics is received much attention as one of the vaccination therapies to compete against the coronavirus disease 2019（COVID-19）pandemic. DDS mRNA therapeutics are generally produced by in vitro transcription utilizing RNA polymerase mediated elongation. However, its purity, stability, and protein synthesis ability, are difficult to be precisely controlled, which is pointed out as drawbacks that must be overcome. To overcome these issues, the introduction of chemically modified nucleic acids is focusing attention. However, it is difficult to flexible molecular design due to the requirement of RNA polymerase recognition ability of chemically modified nucleic acids under in vitro transcription reaction. In the future, the development of a new mRNA design concept based on a flexible molecular design by the progress of chemically modified mRNA therapeutics synthesis method. Under the situation, the authors are focusing on the translation mechanism of mRNA and proposing a new mRNA molecular design to accelerate the translation reaction cycle. In this paper, we introduce an update on therapeutic mRNA design. © 2022, Japan Society of Drug Delivery System. All rights reserved.
RESUMEN
Introductions: In this study, we conducted a training program for preventing COVID-19 in facilities for mentally retarded children by using a participatory approach, with our aim to explore the impacts on the training program. Method: New action tools were developed through three steps. First, we reviewed relevant references and collected existing action tools related to COVID-19 respond. Then we visited the facilities and collected good practices for preventing COVID-19. Finally, we discussed the practical ways and requirements for action tools effective in the facilities with disabilities. We conducted a two-hour program for 4 times at the facilities. The program was consisted of short lecture about COVID-19 and group discussions based on action checklist exercise. Results: The staff members reviewed existing measures at the facilities and discussed point to be improved about COVID-19 preventing. In addition, they shared their concerns and doubts about COVID-19 measures that they felt in their daily tasks during group discussions. They felt difficulty, because of the children with disability sometimes were not able to use face masks, wash your hands and gargle regularly to prevent infection. However, many good practices for standard precaution adapted for disabled children were shared through the group discussions. Discussion: By using a participatory approach that emphasizes social dialogue and action-oriented rather than one-way lecture-type training, staff members empower multifaceted actions for prevention COVID-19 with their own initiatives.